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This project addresses two grand challenges—cutting-edge STEM content and K-12 science assessments. Using DNA Sequencing Analysis Program (DSAP), which will be modified, high school students and teachers will learn molecular biology and modern genetics by working with authentic genomic sequences, and submit their findings for review by scientists. The objective is to develop state-of-the-art Web-based tools and resources that will make it possible for high school students to conduct authentic research in bioinformatics.